纳米金催化Luminol-AgNO_3化学发光体系测定盐酸阿米替林

碱性条件下,纳米金对Luminol-Ag NO3化学发光体系有增敏作用,盐酸阿米替林对该化学发光体系有显著的增敏作用。基于此,在优化化学发光反应条件的基础上,提出了测定盐酸阿米替林的新方法,并对其化学发光机理进行了探讨。该法测定盐酸阿米替林的线性范围为3.0×10-9~3.0×10-7g/m L,相关系数(r)为0.999 4,检出限(S/N=3)为2.1×10-9g/m L,相对标准偏差(RSD)为2.0%(n=11,ρ盐酸阿米替林=5.0×10-8g/m L)。该法已成功用于药物制剂中盐酸阿米替林含量的测定。

Determination of Amitriptyline Hydrochloride by Gold Nanoparticles Catalyzed Luminol-Silver Nitrate System

Under alkaline condition, gold nanoparticles(AuNPs) could enhance the chemiluminesence(CL) of luminol-silver nitrate system,and amitriptyline hydrochloride could greatly enhance CL signals. Based on this,a new simple CL method was developed to detect the content of amitriptyline hydrochloride.The experimental conditions were optimized,and the possible mechanism of the system was discussed.Under the optimal conditions,the relative CL intensity was linearly proportional to amitriptyline hydrochloride concentration in the range of 3.0×10-9-3.0×10-7 g/mL,with a correlation coefficient of 0.999 4 and a detection limit(S/N=3) of 2.1×10-9 g/mL.The RSD (n=11) was 2.0% for 5.0×10-8 g/mL amitriptyline hydrochloride.The method was applied in the determination of amitriptyline hydrochloride tablets,and the result was in consistent with the standard method.