Simple enzyme reactors suitable for the byproduct-free preparation of the aglycones of naturally occurring glycosides under mild conditions |
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Authors: | P Turecek F Pittner |
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Institution: | 1. Institut f. Allgemeine Biochemie der Univ., Wien 2. Ludwig Boltzmann Forschungsstelle, W?hringerstr. 38, A - 1090, Wien, Austria
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Abstract: | Naringinase from Penicillium species—containing α-l-rhamnosidase and β-d-glucosidase activity—immobilized on silicate carriers can be used as a mild and effective means to cleave naturally occurring
glycosides into their aglycones and sugar components. The procedure was tested with flavonoid-, anthraquinone-, and steroidglycosides.
Since the solubility of such compounds is limited in aqueous solutions, a simple batch procedure has been developed to convert
solid substrates suspended in only small amounts of buffer to the desired products with high yields. |
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Keywords: | Immobilized naringinase glycosides aglycones preparation of glycosides cleavage of |
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