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A new spectrophotometric readout for the alanine-triphenyl tetrazolium chloride system for high-dose dosimetry |
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| Authors: | S Ebraheem W B Beshir A Kovacs L Wojnarovits W L McLaughlin |
| Institution: | a National Center for Radiation Research and Technology, P.O. Box 29, Madinat Nasr, Cairo, Egypt b Institute of Isotope and Surface Chemistry, Chemical Research Center, Hungarian Academy of Sciences, P.O. Box 77, H-1525 Budapest, Hungary c Physical Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA |
| Abstract: | The electron spin resonance (ESR) readout of radical concentration in irradiated L-alanine is well known as a transfer dosimetry system, however, the high cost of the ESR equipment is a serious handicap for large-scale routine application. In this study the reaction between irradiated L-alanine powder and aqueous solution of triphenyl tetrazolium chloride (TTC) was studied. The radical produced by irradiation of alanine reacts during the dissolution with TTC forming pink-colored formazans with absorption maxima around 401 and 487 nm. This readout method can be used in the 2–200 kGy dose range. |
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