| HPLC-DAD法测定辣椒及其制品中苏丹红的含量 |
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| 引用本文： | 颜振敏,吴艳兵,南海娟,张永生,魏新军.HPLC-DAD法测定辣椒及其制品中苏丹红的含量[J].光谱实验室,2011,28(1):109-112. |
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| 作者姓名： | 颜振敏 吴艳兵 南海娟 张永生 魏新军 |
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| 作者单位： | 河南科技学院食品学院;河南科技学院资源与环境学院; |
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| 基金项目： | 河南科技学院高层次人才资助项目(070021) |
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| 摘 要： | 建立了应用高效液相色谱测定辣椒粉及其少量含油制品中的苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ含量的检测方法.采用Symmetry C18反相色谱柱(3.9mm×150mm,5μm),流动相为乙腈醋酸溶液(V乙磷:Vo.1%醋酸＝7∶1),等度洗脱,流速1.0mL/min;柱温30℃;检测波长230nm.在选定色谱条件下,苏丹红Ⅰ、Ⅱ、Ⅲ、...
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| 关 键 词： | 苏丹红 高效液相色谱 二极管阵列检测器 |
Determination of Sudan Red in Chilli and Related Products by HPLC-DAD |
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| YAN Zhen-Min,WU Yan-Bing,NAN Hai-Juan,ZHANG Yong-sheng,WEI Xin-Jun.Determination of Sudan Red in Chilli and Related Products by HPLC-DAD[J].Chinese Journal of Spectroscopy Laboratory,2011,28(1):109-112. |
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| Authors: | YAN Zhen-Min WU Yan-Bing NAN Hai-Juan ZHANG Yong-sheng WEI Xin-Jun |
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| Institution: | YAN Zhen-Min WU Yan-Bing a NAN Hai-Juan ZHANG Yong-Sheng WEI Xin-Jun(School of Food Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003,P.R.China)a(School of Resource and Environment,P.R.China) |
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| Abstract: | The method for determination of Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ in chilli and related products by reversed-phase high performance liquid chromatography was developed.The separation was performed on symmetry C18 column（3.9mm×150mm,5μm）by equal degree elution using acetonitrile’s acidic water as the mobile phase.The flow rate was 1.0mL/min with 230nm as detection wavelength.The results showed that Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ have good linearity in the range from 0 to 5 mg/L.The relative standard deviations of spiked sample were 2.58%,2.25%,2.65% and 2.39%.The detection limits of four Sudan dyes were 20,40,40μg/kg and 60μg/kg.The average recovery of four Sudan Red dyes was 98.67%—100.67%.This method is rapid,sensitive,simple and accurate. |
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| Keywords: | Sudan Red High Performance Liquid Chromatography Diode Array Detector |
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