| A Novel Method for Fabrication of a Glass-Electrode-Based Lipase Sensor |
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| 作者姓名： | Xi Rong HUANG * Yue Zhong LI Guo Liang YANG Li Li LIU Yin Bo QU Wen Juan ZHANG State Key Lab of Microbial Technology of China Shandong University Jinan Key Lab for Colloid and Inferface Chemistry of the Education Ministry of Chin |
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| 作者单位： | Xi Rong HUANG1，2*，Yue Zhong LI1，Guo Liang YANG1，Li Li LIU2，Yin Bo QU1，Wen Juan ZHANG2 1State Key Lab of Microbial Technology of China，Shandong University，Jinan 250100 2 Key Lab for Colloid and Inferface Chemistry of the Education Ministry of Chin |
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| 摘 要： | Lipase is one of the hydrolases. It catalyzes the hydrolysis of triglycerides, liberating fat acids. Based on its catalysis and the response of a glass electrode to the acids, a glass-electrode-based lipase sensor can be fabricated1, which has great potential in clinical medicine for the detection of blood fat in human serum samples.The immobilization of lipase on the bulb of a glass electrode is the key to the development of the sensor. In addition to its response time, which has a lot to do …
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A Novel Method for Fabrication of a Glass-Electrode-Based Lipase Sensor |
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| Xi Rong HUANG,*,Yue Zhong LI,Guo Liang YANG,Li Li LIU,Yin Bo QU,Wen Juan ZHANG State Key Lab of Microbial Technology of China,Shandong University,Jinan Key Lab for Colloid and Inferface Chemistry of the Education Ministry of Chin.A Novel Method for Fabrication of a Glass-Electrode-Based Lipase Sensor[J].Chinese Chemical Letters,2001(5). |
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| Authors: | Xi Rong HUANG Yue Zhong LI Guo Liang YANG Li Li LIU Yin Bo QU Wen Juan ZHANG State Key Lab of Microbial Technology of China Shandong University Jinan Key Lab for Colloid and Inferface Chemistry of the Education Ministry of Chin |
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| Institution: | Xi Rong HUANG1,2*,Yue Zhong LI1,Guo Liang YANG1,Li Li LIU2,Yin Bo QU1,Wen Juan ZHANG2 1State Key Lab of Microbial Technology of China,Shandong University,Jinan 250100 2 Key Lab for Colloid and Inferface Chemistry of the Education Ministry of Chin |
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| Abstract: | Based on an unusual reversible sol-gel transition phenomenon, a novel method for the fabrication of a lipase electrode was developed. The response characteristics of the biosensor was studied by potentiometric technique using olive oil as substrate. After optimization, the lipase electrode demonstrated high activity and good stability. |
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| Keywords: | Lipase microemulsion-based gel biosensor response characteristics |
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