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| 高效液相色谱法测定血红蛋白氧载体中残留的戊二醛 | |
| 引用本文： | 严坤平,景小丹,韩静,但宁,陈超.高效液相色谱法测定血红蛋白氧载体中残留的戊二醛[J].分析化学,2009,37(10). |
| 作者姓名： | 严坤平 景小丹 韩静 但宁 陈超 |
| 作者单位： | 1. 西北大学生命科学学院,西安,710069;陕西北美基因股份有限公司,西安,710069 2. 陕西北美基因股份有限公司,西安,710069 3. 西北大学生命科学学院,西安,710069;国家微检测工程研究中心,西安,710069 |
| 基金项目： | 国家"863计划"基金 |
| 摘 要： | 建立了一种测定血红蛋白氧载体中戊二醛残余含量的高效液相色谱方法.用10 kDa超滤膜通过离心3000 r/min×20 min将游离戊二醛从待测样品中分离;在pH 1.0时,在含有70%乙腈的体系中用2,4-二硝基苯肼在30 min内将戊二醛衍生成2,4-二硝基苯腙(n(2,4-二硝基苯肼): n(戊二醛)=60: 1),以70%乙腈为流动相,避免了衍生产物生成沉淀.用高效液相色谱法分离测定衍生物,可在20 min内完成分离,同时对衍生步骤进行了优化.本方法具有较高的灵敏度和良好的线性关系,检出限为1.0 ng,在0.1～10 mg/L范围内其线性相关系数为0.9999,重复测定6次的相对标准偏差小于3.0%,回收率为95.26%. |
| 关 键 词： | 戊二醛 高效液相色谱 血红蛋白氧载体 4-二硝基苯肼 |
Detection of Residual Glutaraldehyde in Hemoglobin-Based Oxygen Carrier with High Performance Liquid Chromatography |
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| YAN Kun-Ping,JING Xiao-Dan,HAN Jing,DAN Ning,CHEN Chao.Detection of Residual Glutaraldehyde in Hemoglobin-Based Oxygen Carrier with High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,2009,37(10). | |
| Authors: | YAN Kun-Ping JING Xiao-Dan HAN Jing DAN Ning CHEN Chao |
| Institution: | YAN Kun-Ping1,2,JING Xiao-Dan2,HAN Jing2,DAN Ning1,CHEN Chao1,31(College of Life Science,Northwest University,Xi'an 710069) 2(Shaanxi Lifegen Co.,Ltd.,Xi'an 710069)3(National Engineering Research Center for Miniaturized Detection System,Xi'an 710069) |
| Abstract: | A method for the determination of glutaraldehyde in hemoglobin-based oxygen carrier(HBOC) by high performance liquid chromatography is described.Free glutaraldehyde was separated from HBOC by 10 kDa ultrafiltration membrane under centrifuged 3000 r/min for 20 min and derivatized under the conditions of pH=1.0,2,4-dinitrophenylhydrazine(DNPH):glutaraldehyde=60:1,reaction time 30 min in 70% acetonitrile medium.Precipitation of derivatives of glutaraldehyde with DNPH was avoided under this condition and it was... |
| Keywords: | 2 |
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