Determination of catecholamines and indoleamines in human urine based on intramolecular excimer-forming derivatization and fluorescence detection. |
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Authors: | Hideyuki Yoshida Fumito Kido Makoto Yoshitake Kenichiro Todoroki Hitoshi Nohta Masatoshi Yamaguchi |
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Institution: | Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan. |
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Abstract: | A liquid chromatographic (LC) determination of catecholamines and indoleamines is described. This is based on intramolecular excimer-forming fluorescence derivatization with 4-(1-pyrene)butanoyl chloride, followed by reversed-phase LC. The analytes, containing an amino moiety and phenolic hydroxyl moieties in a molecule, were converted to the corresponding polypyrene-labeled derivatives by one-step derivatization. They afforded intramolecular excimer fluorescence, which can clearly be discriminated from the normal fluorescence emitted from reagent blanks. The detection limits (S/N = 3) for catecholamines and indoleamines were femto-mole levels per 20-microL injection. Furthermore, this method was applied to a urine assay. |
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