High-frequency EPR studies on cofactor radicals in photosystem I |
| |
Authors: | C Teutloff W Hofbauer S G Zech M Stein R Bittl W Lubitz |
| |
Institution: | 1. Max-Volmer-Laboratorium, Technische Universit?t Berlin, Berlin, Germany 2. Institut für Experimentalphysik, Freie Universit?t Berlin, Berlin, Germany 3. Max-Planck-Institut für Strahlenchemie, Mülheim/Ruhr, Germany
|
| |
Abstract: | Electron paramagnetic resonance (EPR) spectroscopy at W-band (94 GHz) is used to resolve theg-tensors of the radical ions of the primary chlorophyll donor P 700 +? and the quinone acceptor A 1 ?? in photosystem I. The obtainedg-tensor principal values are compared with those of the isolated pigment radicals in organic solvents and the shifts are related to an impact of the protein environment. P 700 +? has been investigated in photosystem I single crystals at 94 GHz. W-band EPR applied to the photoinduced radical pair P 700 +? A 1 ?? is used to correctly assign theg-tensor axes of P 700 +? to the molecular structure of the primary donor. Density functional theory calculations on a model of A 1 ?? in its binding pocket derived from the recent crystal structure of photosystem I were utilized to correlate experimental magnetic resonance parameters with structural elements of the protein. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|