弱直流电场对枯草芽孢杆菌生长和代谢活性的作用

枯草芽孢杆菌63501(Bacillus subtilis 63501)培养液在连续培养时分别通入不同强度的直流电场,研究了它们在持续直流电刺激下的生长曲线、pH曲线、ATP酶活性、胞内总蛋白含量、细胞膜通透性以及细菌微观形态变化。结果表明,适宜的直流电能促进枯草芽孢杆菌增殖;枯草芽孢杆菌的ATP酶活性在0.0455×10-3A/cm2电流密度作用下,在对数生长前期、后期和稳定期分别比对照组同期提升1.9、3.5和3.8倍,表明适宜强度的电流增强了细菌细胞的代谢活性。细菌通透性也有不同程度的提高,与透射电子显微镜观察到的细菌细胞通电后的形态变化具有一致性。

Effects of Low Direct Current Electric Field on the Growth and Metabolism of Bacillus subtilis

The growth and metabolism of Bacillus subtilis 63501 were investigated under low direct current(DC) electric filed.The effects of the electric filed on the growth,pH values,ATPase activity,total amount of protein and cells membrane penetration of Bacillus subtilis 63501 solutions were studied.The surface morphologies of the bacteria were also characterized by transimission electronic microscopy(TEM).The results indicated that the growth of such bacteria could be enhanced under an appropriate DC electric field.The ATPase activity of Bacillus subtilis 63501 could be multiplied by 1.9,3.5 and 3.8 times during the control in prophase,anaphase and stable phase under 0.0455×10-3 A/cm2 current density,suggesting that suitable direct current density can promote the metabolism activity of Bacillus subtilis 63501,which is consistent with the results form TEM observations.