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Ionic liquid; possibility of protein-preserving solvent under high pressure
Authors:Machiko Shigemi  Yukihiro Yoshimura
Affiliation:Department of Applied Chemistry , National Defense Academy , 1-10-20, Hashirimizu, Yokosuka , Kanagawa , 239-8686 , Japan
Abstract:We have investigated the pressure-induced phase transition behavior (~3.0 GPa) of aqueous 1-butyl-3-methylimidazolium chloride ([bmim][Cl]) solutions with N-methylacetamide (NMA), which is a simple protein model compound, using Raman spectroscopy. From Raman spectral changes and optical observation in the sequence of elevated pressure, we found that the aqueous [bmim][Cl] solution with NMA in the water-rich condition induces the high pressure crystallization at 2.6 GPa. On the other hand, in the [bmim][Cl]-rich condition, high pressure crystalline phase was not observed even up to 3.0 GPa. Our results show that the aqueous [bmim][Cl] solution in the ionic liquid-rich condition along with the use of pressure has a potential for protein-preserving solvent.
Keywords:protein storage  phase transition  ionic liquids  Raman spectroscopy
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