分子信标研究5-氟尿嘧啶对鼻咽癌HNE1细胞p33ING1基因转录水平的影响

p33ING1作为一种重要的抑癌基因，在乳腺癌及胃癌等恶性肿瘤细胞中的mRNA表达水平显著低于癌旁正常组织细胞中的表达，并导致这些肿瘤细胞的侵袭转移能力增强，对化疗药物及r射线和紫外线处理的敏感性降低。

Investigation of the Effect of 5-FU on p33ING1 mRNA Level Based on Molecular Beacon

In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33~(ING1) mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33~(ING1) were measured in vitro.The results were as follows: p33~(ING1) mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.The MTT results also proved that high expression of p33~(ING1) mRNA can increase cell's sensitivity to chemical drug 5-FU.The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.