高效液相色谱表征高聚物*

最常用的测试高聚物的分子量和分子量分布的体积排除色谱(SEC)是高效液相色谱 (HPLC)的一个重要分支，HPLC的另一个重要分支是相互作用液相色谱, 它是20世纪90年代开始用于高分子分离和表征的研究领域。相互作用液相色谱可以根据高分子的化学结构（如共混物组成、共聚物组成、端基）来分离，它比SEC 有更高的分离效率。本文介绍了高聚物液相色谱的分离模式，并就高聚物体积排除色谱、相互作用液相色谱、临界液相色谱和全二维液相色谱用于分离和表征高聚物的研究进展进行了较系统的综述，并对该技术目前存在的问题和今后可能的发展前景进行了探讨。

Characterization of Polymers by High Performance Liquid Chromatography

Size-exclusion chromatography (SEC) is the most commonly applied high performance liquid chromatography (HPLC) technique separating molecules based on the size for the measurement of the molecular-weight averages and the molecular-weight distribution (MWD) for polymers. Interactive HPLC, which is based on molecular interactions between the polymer molecules and the mobile and stationary phases in the column, can be used to separate polymers based on chemical composition or functionality (functional groups or end-groups). Interactive LC is superior when compared to SEC, with respect to selectivity and peak capacity. In this review, the different HPLC modes are introduced for separation and characterization of polymers. The progress on SEC, interactive HPLC, critical liquid chromatography and comprehensive two dimensional LC of polymers is summarized. The limitations and prospective applications of the technique for separation and characterization of polymers are also discussed. Contents
1 Introduction
2 Separation modes in liquid chromatography of polymers
3 Size-exclusion chromatography of polymers
4 Interactive liquid chromatography of polymers
5 Critical liquid chromatography of polymers
6 Comprehensive two dimensional liquid chromatography of polymers
7 Outlook