| SELECTION OF RECOMBINANT VACCINIA VIRUSES (TIAN TAN STRAIN) EXPRESSING HEPATITIS B VIRUS SURFACE ANTIGEN BY USING β-GALACTOSIDASE AS A MARKER* |
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| 引用本文： | 刘庚起,曹旭,朱既明,任贵方,李红梅,解燕乡,彭薇.SELECTION OF RECOMBINANT VACCINIA VIRUSES (TIAN TAN STRAIN) EXPRESSING HEPATITIS B VIRUS SURFACE ANTIGEN BY USING β-GALACTOSIDASE AS A MARKER*[J].中国科学B辑(英文版),1990(2). |
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| 作者姓名： | 刘庚起 曹旭 朱既明 任贵方 李红梅 解燕乡 彭薇 |
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| 作者单位： | Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC，Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC，Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC，Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC，Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC，Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC，Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052，PRC |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | We constructed a plasmid that contains a small piece of DNA with two vaccinis promoters running in opposite directions——a promoter from a late gene encoding an 11 K polypeptide (P11) and a promoter from an early gene encoding 25K(P25). These promoters were isolated from the Tian Tan strain of vaccinia virus and were flanked by the thymidine kinase (TK) sequence of the same virus. Genes encoding the hepatitis B virus surface antigen (HBsAg) and the Rscherichia coli β-galactosidase (LacZ) were inserted downstream of the 11 K and 25 K promoters respectively so that coexpression plasmids were constructed. Recombinant vaccinis Viruses were selected directly by picking blue plaques formed under overlaying agarose medium containing X-gal. HBsAg was expressed to high level by these recombinant viruses. These recombinant viruses showed reduced virulence on rabbit skin and induced anti-HBs after intrsdermal inoculation of rabbits.
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| 关 键 词： | recombinant vaccinia viruses hepatitis B β-galactosidase |
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