Quantitative determination of alpha(s2)- and alpha(s1)-casein in goat's milk with different genotypes by capillary electrophoresis |
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Authors: | Gómez-Ruiz José Angel Miralles Beatriz Agüera Pastora Amigo Lourdes |
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Institution: | Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain. |
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Abstract: | A capillary electrophoresis (CE) method has been applied for the quantitative determination of alpha(s1)- and alpha(s2)-CN in goat's milk. Several analytical parameters were evaluated showing the reliability of this CE method. Coefficients of determination (R2) greater than 99% were obtained and determination limits of 1.23 and 0.98 mg/ml were achieved for alpha(s1)- and alpha(s2)-CN, respectively. The analytical parameters studied in terms of accuracy, precision and recovery were within acceptable limits. Among 18 samples of 4 different genotypes (BB, EE, BF and FF) for alpha(s1)-CN were analysed, different amounts were obtained from the genotypes. |
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