Semicarbazide-functionalized Si(111) surfaces for the site-specific immobilization of peptides |
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Authors: | Coffinier Y Olivier C Perzyna A Grandidier B Wallart X Durand J-O Melnyk O Stiévenard D |
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Institution: | Institut d'Electronique et de Microélectronique et de Nanotechnologie, IEMN, (CNRS, UMR 8520) Département ISEN, 41 bd Vauban, 59046 Lille Cedex, France. |
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Abstract: | The covalent attachment of semicarbazide-functionalized layers to hydrogen-terminated Si(111) surfaces is reported. The surface modification, based on the photoinduced hydrosilylation of a Si(111) surface with protected semicarbazide-functionalized alkenes, was investigated by means of X-ray photoelectron spectroscopy (XPS), contact angle measurements, and atomic force microscopy (AFM). The removal of the protecting group yielded a semicarbazide-terminated monolayer which was reacted with peptides bearing a glyoxylyl group for site-specific alpha-oxo semicarbazone ligation. |
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