| 强极性中药组分的亲水作用色谱保留行为 |
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| 引用本文： | 韩超,戴小军,雷根虎,卫引茂. 强极性中药组分的亲水作用色谱保留行为[J]. 分析化学, 2010, 38(11). DOI: 10.3724/SP.J.1096.2010.01615 |
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| 作者姓名： | 韩超 戴小军 雷根虎 卫引茂 |
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| 作者单位： | 西北大学合成与天然功能分子化学教育部重点实验室,化学与材料科学学院,西安,710069;西北大学合成与天然功能分子化学教育部重点实验室,化学与材料科学学院,西安,710069;西北大学合成与天然功能分子化学教育部重点实验室,化学与材料科学学院,西安,710069;西北大学合成与天然功能分子化学教育部重点实验室,化学与材料科学学院,西安,710069 |
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| 基金项目： | 国家自然科学基金(Nos.20975080,20875075); 教育部新世纪优秀人才支持计划项目(No.NCET-08-0892)资助 |
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| 摘 要： | 采用亲水作用色谱模式,以二醇基硅胶为固定相,水-有机溶剂为流动相,通过改变流动相中有机溶剂种类及浓度、缓冲盐、有机酸种类及其浓度、柱温等条件,研究了强极性中药组分在亲水作用色谱中的保留行为。结果表明:流动相中水的比例在0～100%(V/V)变化时,溶质保留呈U型曲线,属于亲水色谱和反相色谱的混合保留机理。水含量在0～50%(V/V)范围时,亲水作用控制溶质的保留。溶质保留随流动相中缓冲盐浓度的增大而增强。对于酸性溶质,其保留随有机酸三氟乙酸、甲酸、乙酸的酸性降低而增大。
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| 关 键 词： | 亲水作用色谱 二醇色谱柱 强极性化合物 保留行为 |
Retention Behaviors of Strongly Polar Compounds from Traditional Chinese Medicine in Hydrophilic Interaction Chromatography |
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| HAN Chao,DAI Xiao-Jun,LEI Gen-Hu,WEI Yin-Mao. Retention Behaviors of Strongly Polar Compounds from Traditional Chinese Medicine in Hydrophilic Interaction Chromatography[J]. Chinese Journal of Analytical Chemistry, 2010, 38(11). DOI: 10.3724/SP.J.1096.2010.01615 |
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| Authors: | HAN Chao DAI Xiao-Jun LEI Gen-Hu WEI Yin-Mao |
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| Affiliation: | HAN Chao,DAI Xiao-Jun,LEI Gen-Hu,WEI Yin-Mao* ( Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education,College of Chemistry & Material Science,Northwest University,Xi'an 710069) |
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| Abstract: | The retention behaviors of six polar compounds from traditional Chinese medicine were investigated with hydrophilic interaction chromatography ( HILIC) by changing kinds of organic solvent and concentration, additives ( salt and organic acid) in mobile phase and column temperature. The retention curves of six solutes exhibitUshape with the change in the concentration of water from 0 to 100% ( V/V) in mobile phase, indicating a mixed-mode of hydrophilic interaction and reversed phase chromatography. The rete... |
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| Keywords: | Hydrophilic interaction chromatography Diol column Strongly polar compounds Retention behavior |
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