离线二维液相色谱法分离巴天酸模根的化学成分

建立了离线二维反相/反相液相色谱分离体系（2D-RPLC/RPLC），对巴天酸模中的化学成分进行分离。通过比较巴天酸模乙酸乙酯萃取液在环氧四氮唑和Unitary C18色谱柱上的高效液相色谱图，确定以环氧四氮唑色谱柱为第一维色谱柱，以Unitary C18色谱柱为第二维色谱柱。流动相均采用0.1%（v/v）甲酸水溶液和甲醇，梯度洗脱。经一维色谱分离后，共收集18个流分，采用二维色谱对这18个流分进行了进一步的分离分析。实验结果表明，该二维色谱分离方法高效、可行，为巴天酸模药材的微量组分的分离以及活性化合物的筛选提供了分离方法。

Separation of chemical compositions in root of Rumex patientia L. with off-line two-dimensional liquid chromatography

An innovative analytical method based on the off-line two-dimensional reversed-phase/reversed-phase liquid chromatography (2D-RPLC/RPLC) was established to separate the components of root of Rumex patientia L. The chromatograms of ethyl acetate extract of root of Rumex patientia L. on a phenyl/tetrazolium column (250 mm×4.6 mm, 7 μm) and a Unitary C18 column (250 mm×4.6 mm, 7 μm) were compared, and they showed different separation abilities. A phenyl/tetrazolium column was used for the first dimensional separation with 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases, and 18 fractions was collected. The second dimensional liquid chromatography analysis was carried out on a Unitary C18 column with 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases. Based on the experiment setup and results, it was concluded that off-line 2D-LC can be an effective method for the separation of the trace components and the screening of active compounds of root of Rumex patientia L.