Preliminary study to prepare a reference material of styrene metabolites – mandelic acid and phenylglyoxylic acid – in human urine |
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Authors: | I ?perlingová L Dabrowská V Stránský J Kuˇcera M Tichý |
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Institution: | (1) National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic e-mail: sperling@szu.cz Tel.: +420-2-6708 2675 Fax: +420-02-6731 12 36, CZ;(2) Nuclear Physics Institute, 250 68 Řeˇz near Prague, Czech Republic, CZ |
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Abstract: | A preliminary batch of the reference material was prepared by freeze-drying pooled urine samples obtained from healthy persons
occupationally exposed to styrene. Tests for homogeneity and stability were performed by determining urine concentrations
of mandelic (MA) and phenylglyoxylic acids (PGA). The urinary MA and PGA concentrations were followed over an 8-month period
using high performance liquid chromatography (HPLC). No changes of the concentration values were found. Pure PA and PGA from
Merck and Fluka, respectively, were used for traceability purposes, because certified or standard reference materials for
MA and PGA do not exist. Control material ClinChek-Urine Control (Recipe) was analysed simultaneously. The mean values of
MA and PGA compared well with the means of control samples and fell within the control range. The certified values and their
uncertainties were evaluated from the results of interlaboratory comparisons, homogeneity (277.0 ± 7.4 mg L−1 for MA and 148.0 ± 4.7 mg L−1 for FGA) and stability tests. The values are unweighted arithmetical averages of accepted results and their uncertainties
are combined uncertainties enlarged by coefficient k=1, evaluated from the standard uncertainties of the interlaboratory comparison, homogeneity and stability tests.
Received: 17 September 2002 Accepted: 1 November 2002
Acknowledgement This work was supported by the Internal Grant Agency of Ministry of Health of the Czech Republic (Grant NJ/6784–3).
Presented at CERMM-3, Central European Reference Materials and Measurements Conference: The function of reference materials
in the measurement process, May 30–June 1, 2002, Rogaška Slatina, Slovenia
Correspondence to I. Šperlingová |
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Keywords: | Reference material Styrene metabolites Uncertainty |
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