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A Dual 13C and 15N Long Term Labelling Technique to Investigate Uptake and Translocation of C and N in Beech (Fagus sylvatica L.)
Authors:J. Dyckmans  H. Flessa  Z. Shangguan  F. Beese
Affiliation:1. Institute of Soil Science and Forest Nutrition, University of G?ttingen , Germany;2. National Laboratory of Soil Erosion and Dryland Farming , Institute of Soil and Water Conservation, Chinese Academy of Sciences , Yangling, Shaanxi, 712100, P.R. China
Abstract:Abstract

A continuous dual 13CO2 and 15NH4 15NO3 labelling experimental set-up is presented that was used to investigate the C and N uptake and allocation within 3-year old beech (Fagus sylvatica L.) during one growing season. The C and N allocation pattern was determined after six, twelve and eighteen weeks of growth. The carbon uptake was distinctly different in the three phases examined: The first six weeks after budbreak were dedicated to leaf growth with a R/S (root to shoot) ratio of 0.14 for the new carbon. The second growth phase showed a balanced R/S ratio of C allocation and after week 13, the root compartment was the main carbon sink (R/S = 6.97).

Nitrogen allocation was more basipetal as compared to carbon. In the second growth phase, R/S of Nnew was 5.57 but fell to 3.54 for the third growth phase probably due to formation of reserves in buds and stem.
Keywords:Carbon allocation  carbon 13  Fagus sylvatica  growth chambers  nitrogen allocation  nitrogen 15  nutrient uptake
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