| 双阳离子咔唑衍生物与DNA相互作用的光谱研究 |
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| 引用本文： | 张元红,孙渝明,刘鑫,赵宁,于晓强,黄柏标.双阳离子咔唑衍生物与DNA相互作用的光谱研究[J].高等学校化学学报,2010,31(9):1860. |
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| 作者姓名： | 张元红 孙渝明 刘鑫 赵宁 于晓强 黄柏标 |
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| 作者单位： | 1. 山东大学晶体材料国家重点实验室,
2. 山东大学光学系, 济南 250100 |
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| 摘 要： | 合成了一种新型的双阳离子咔唑衍生物3,6-双(1-羟乙基-4-烯基吡啶)咔唑碘盐(3,6-BHVC), 并对其进行了表征. 利用紫外-可见吸收光谱、圆二色谱、单光子和双光子荧光发射光谱探讨了该化合物与DNA的结合方式. 所有光谱测试结果表明, 该化合物在磷酸根]/染料]比值较低的条件下主要以插入方式与DNA结合, 在该比值较高的条件下主要以沟槽结合方式为主. 表明咔唑衍生物可以成为一种探索具有高结合能力双光子DNA探针的基本结构.
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| 关 键 词： | 双阳离子咔唑衍生物 双光子细胞核荧光探针 结合方式 |
| 收稿时间： | 2010-06-07 |
Interaction Between Dicationic Carbazole Derivatives and Nucleic Acid by Spectrum |
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| ZHANG Yuan-Hong,SUN Yu-Ming,LIU Xin,ZHAO Ning,YU Xiao-Qiang,HUANG Bai-Biao.Interaction Between Dicationic Carbazole Derivatives and Nucleic Acid by Spectrum[J].Chemical Research In Chinese Universities,2010,31(9):1860. |
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| Authors: | ZHANG Yuan-Hong SUN Yu-Ming LIU Xin ZHAO Ning YU Xiao-Qiang HUANG Bai-Biao |
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| Institution: | 1. State Key Laboratory of Crystal Materials, ;
2. Optics Department, Shandong University, Jinan 250100, China |
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| Abstract: | In this paper, 3,6-bis(1-hydroxyethyl-4-vinylpyridinium) carbazole diiodide(3,6-BHVC), a novel dicationic carbazole derivative, was synthesized and characte-rized. Using electronic absorption spectra, circle dichroism spectra and one- and two-photon emission spectra, the binding modes between 3,6-BHVC and DNA were studied. All spectral results show that 3,6-BHVC intercalates into DNA base pairs at lower ratio of phosphate]/3,6-BHVC] and binds in DNA groove at higher ratio. This indicates that carbazole derivative can act as a primary structure to explore two-photon DNA probes with high binding affinity. |
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| Keywords: | Dicationic carbazole derivative Two-photon fluorescent DNA probe Binding mode |
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