Quantification of asenapine and three metabolites in human plasma using liquid chromatography-tandem mass spectrometry with automated solid-phase extraction: application to a phase I clinical trial with asenapine in healthy male subjects |
| |
Authors: | de Boer Theo Meulman Erik Meijering Henri Wieling Jaap Dogterom Peter Lass Holger |
| |
Institution: | QPS Netherlands, Petrus Campersingel 123, 9713 AG Groningen, The Netherlands. theo.de.boer@qps.com |
| |
Abstract: | The development and validation of methods for determining concentrations of the antipsychotic drug asenapine (ASE) and three of its metabolites N-desmethylasenapine (DMA), asenapine-N(+) -glucuronide (ASG) and 11-O-sulfate-asenapine (OSA)] in human plasma using LC-MS/MS with automated solid-phase extraction is described. The three assessment methods in human plasma were found to be acceptable for quantification in the ranges 0.0250-20.0 ng/mL (ASE), 0.0500-20.0 ng/mL (DMA and OSA) and 0.250-50.0 ng/mL (ASG). |
| |
Keywords: | asenapine quantification plasma liquid chromatography–tandem mass spectrometry |
本文献已被 PubMed 等数据库收录! |