Selective Interaction Between Proteins and the Outermost Surface of Polyelectrolyte Multilayers: Influence of the Polyanion Type,pH and Salt |
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| Authors: | Martin Müller Theresia Rieser Paul L Dubin Klaus Lunkwitz |
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| Institution: | 1. Kalle Nalo GmbH & Co KG, Rheingaustrasse 190–196, D-65025 Wiesbaden, Germany;2. Indiana University – Purdue University at Indianapolis, Indianapolis, Indiana 46202, USA;3. Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany;4. Fax: 0351/465 82 84 |
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| Abstract: | Protein adsorption was studied by in-situ ATR-FT-IR spectroscopy of consecutively deposited polyelectrolyte multilayer systems terminated either with poly(ethyleneimine) (PEI) or polyanions, such as poly-(acrylic acid) (PAC), poly(maleic acid-co-propylene) (PMA-P) or poly(vinyl sulfate) (PVS). The influence of the polyanion type, pH and ionic strength was investigated. Negatively charged human serum albumin (HSA) was strongly repelled by multilayers terminated with weak polyanions (PAC, PMA-P), whereas moderate attraction was observed for those terminated with the strong polyanion PVS. Changing the pH from 7.4 to 5 resulted in enhanced HSA adsorption onto PAC-terminated multilayers. An increase in ionic strength diminished the attractive HSA adsorption onto PEI-terminated multilayers. For the PEI/PAC system, the biomedically relevant adsorption of human fibrinogen (FGN) is determined via its isoelectric point in accordance with three other proteins. |
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