Simultaneous Determination of Olanzapine and Fluoxetine by HPLC |
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Authors: | B Venkateswara Reddy K V N Suresh Reddy J Sreeramulu G V Kanumula |
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Institution: | (1) Department of Chemistry, Sri Krishnadevaraya University, Anantapur, 515003, AP, India;(2) Department of Chemistry, Sri Venkateswara University, Tirupati, 517502, AP, India |
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Abstract: | A rapid, specific reversed phase HPLC method has been developed for simultaneous determination of olanzapine and fluoxetine
in their formulations. Chromatographic separation of these two pharmaceuticals was carried out on an Inertsil C18 reversed phase column (150 mm × 4.6 mm, 5 μm) with a 40:30:30 (v/v/v) mixture of 9.5 mM sodium dihydrogen phosphate (pH adjusted to 6.8 ± 0.1 with triethylamine), acetonitrile and methanol as
mobile phase. The flow rate 1.2 mL min−1 and the analytes are monitored at 225 nm. Paroxetine was used as internal standard. The assay results were linear from 25
to 75 μg mL−1 for olanzapine (r
2 ≥ 0.995) and 100–300 μg mL−1 for fluoxetine (r
2 ≥ 0.995), showed intra- and inter-day precision less than 1.0%, and accuracy of 97.7–99.1% and 97.9–99.0%. LOQ was 0.005
and 0.001 μg mL−1 for olanzapine and fluoxetine, respectively. Separation was complete in less than 10 min. Validation of the method showed
it to be robust, precise, accurate and linear over the range of analysis. |
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Keywords: | Column liquid chromatography Pharmaceutical formulations Olanzapine and fluoxetine |
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