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| 合成多肽的电喷雾质谱研究 | |
| 引用本文： | 白进发,刘志强,刘宁,刘淑莹,姬志鹃,盛树力.合成多肽的电喷雾质谱研究[J].分析化学,2005,33(5):661-664. |
| 作者姓名： | 白进发 刘志强 刘宁 刘淑莹 姬志鹃 盛树力 |
| 作者单位： | 1. 中国科学院长春应用化学研究所新药研究实验室,长春,130022;中国科学院研究生院,北京,100039 2. 中国科学院长春应用化学研究所新药研究实验室,长春,130022 3. 北京市宣武医院药理室,北京,100053 |
| 摘 要： | 利用电喷雾多极串联质谱对3种合成多肽进行了系统的鉴定和分析研究。首先通过全扫描模式测定了其分子量，然后选择M H]^ 或M 2H]^2 离子通过串联质谱(MS／MS)得到碎片离子，采用y离子和b离子互补的方法测定了多肽序列。利用文献数据对这种方法进行了验证，实验结果表明，该方法简便、快速、实用。 |
| 关 键 词： | 电喷雾质谱 多肽 序列测定 |
Identification and Analysis of Polypeptides by Electrospray Ionization Tandem Mass Spectrometry |
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| Bai Jinfa,Liu Zhiqiang,Liu Ning,Liu Shuying,Ji Zhijuan,Sheng Shuli.Identification and Analysis of Polypeptides by Electrospray Ionization Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2005,33(5):661-664. | |
| Authors: | Bai Jinfa Liu Zhiqiang Liu Ning Liu Shuying Ji Zhijuan Sheng Shuli |
| Abstract: | Identification of three polypeptides prepared by solid-phase synthesis using electrospray ionization mass spectrometry is reported. Molecular weights of the polypeptides were determined firstly by full-scan mass spectrometry, then fragmentation ions were obtained by selecting M+H] + or M+2H] 2+ ion using tandem mass spectrometry (MS/MS), the sequence of the polypeptides was confirmed by using complementary y ion and b ion method. The method was further validated by literature data, and it is simple, speed and practical. |
| Keywords: | Electrospray ionization mass spectrometry polypeptide sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |