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| 鬼臼酰肼镍(Ⅱ)金属配合物与DNA的相互作用 | |
| 引用本文： | 王平红,张岐,王流芳,宋玉民,曲建强,刘营前.鬼臼酰肼镍(Ⅱ)金属配合物与DNA的相互作用[J].光谱学与光谱分析,2006,26(5):941-943. |
| 作者姓名： | 王平红 张岐 王流芳 宋玉民 曲建强 刘营前 |
| 作者单位： | 1. 海南大学精细化工海南省重点实验室,海南,海口,570228 2. 兰州大学应用有机国家重点实验室,甘肃,兰州,730000 3. 西北师范大学化学化工学院,甘肃,兰州,730070 |
| 基金项目： | 中国科学院资助项目 , 海南省自然科学基金 |
| 摘 要： | 利用吸收光谱、荧光光谱、DNA热变性研究了鬼臼酰肼金属配合物与小牛胸腺DNA之间的相互作用.在金属配合物的存在下,DNA的紫外吸收光谱产生了明显的减色效应.实验结果表明,鬼臼酰肼金属配合物与DNA之间主要以嵌入方式相结合. |
| 关 键 词： | 金属配合物 溴化乙锭 小牛胸腺DNA |
| 文章编号： | 1000-0593（2006）05-0941-03 |
| 收稿时间： | 2005-03-19 |
| 修稿时间： | 2005-08-20 |
Studies on the Interaction of the Metal Complex of Hydrazide of Podophyllic Acid with DNA |
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| WANG Ping-hong,ZHANG Qi,WANG Liu-fang,SONG Yu-min,QU Jian-qiang,LIU Ying-qian.Studies on the Interaction of the Metal Complex of Hydrazide of Podophyllic Acid with DNA[J].Spectroscopy and Spectral Analysis,2006,26(5):941-943. | |
| Authors: | WANG Ping-hong ZHANG Qi WANG Liu-fang SONG Yu-min QU Jian-qiang LIU Ying-qian |
| Institution: | 1. Province Key Laboratory of Refined Chemistry, Hainan University, Haikou 570228, China;2. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China ;3. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China |
| Abstract: | The interaction between the metal complex of hydrazide of podophyllic acid and calf thymus (CT) DNA was studied by using absorption spectra, fluorescence spectra and DNA heat denaturation. It was found that the intensity of the maximal absorption peaks from absorption spectra is weakened in the presence of the metal complex of hydrazide of podophyllic acid compared with that in the absence of the metal complex. All the experimental results show that the intercalation mode was proved to exist between HDPP-Ni complexes and CT DNA. |
| Keywords: | Metal complex Ethidium bromide Calf thymus DNA |
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