Reductive cleavage mechanism of methylcobalamin: elementary steps of Co-C bond breaking |
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Authors: | Kozlowski Pawel M Kuta Jadwiga Galezowski Wlodzimierz |
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Institution: | Department of Chemistry, University of Louisville, Louisville, KY 40292, USA. pawel@louisville.edu |
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Abstract: | Density functional theory has been applied to the investigation of the reductive cleavage mechanism of methylcobalamin (MeCbl). In the reductive cleavage of MeCbl, the Co-C bond is cleaved homolytically, and formation of the anion radical (MeCbl]*-) reduces the dissociation energy by approximately 50%. Such dissociation energy lowering in MeCbl]*- arises from the involvement of two electronic states: the initial state, which is formed upon electron addition, has dominant pi*corrin character, but when the Co-C bond is stretched the unpaired electron moves to the sigma*Co-C state, and the final cleavage involves the three-electron (sigma)2(sigma*)1 bond. The pi*corrin-sigma*Co-C states crossing does not take place at the equilibrium geometry of MeCbl]*- but only when the Co-C bond is stretched to 2.3 A. In contrast to the neutral cofactor, the most energetically efficient cleavage of the Co-C bond is from the base-off form. The analysis of thermodynamic and kinetic data provides a rationale as to why Co-C cleavage in reduced form requires prior departure of the axial base. Finally, the possible connection of present work to B12 enzymatic catalysis and the involvement of anion-radical-like MeCbl]*- species in relevant methyl transfer reactions is discussed. |
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