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On-Line Sample Treatment LC System for MS Compatibility |
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| Authors: | Yuki Hashi Jian-Guo Yao Yue-Qi Li Yan Liu Jin-Ming Lin |
| Institution: | 1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China 2. Analytical Applications Center, Shimadzu (Hong Kong) Limited, Beijing Office, Beijing, 100020, China 3. Department of Chemistry, Tsinghua University, Beijing, 100084, China |
| Abstract: | In the present work, a new liquid chromatography–mass spectrometry (LC–MS) system with on-line pretreatment using column switching and a dilution function was developed. This system can be used under conventional high-performance liquid chromatography (LC) separation conditions, including mobile phases containing phosphate buffer. The built-in autodilution function greatly improves the trapping efficiency for target compounds, followed by desaltation that optimizes the ionization conditions for MS analysis. This fully automated two-dimensional LC system interfaced with mass spectrometry provided a powerful tool for the determination of impurity profiles in pharmaceutical research and the identification of traditional Chinese medicine in natural products. |
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