Extraction and Determination of Quercetin and Myricetin from Chamaecyparis obtusa by Ionic Liquids‐based Monolithic Cartridge |
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Authors: | Tao Zhu Wentao Bi Kyungho Row |
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Institution: | Department of Chemical Engineering, Inha University, 253 Yonghyun‐Dong, Nam‐Ku, Incheon 402‐751, Korea |
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Abstract: | A short ionic liquids (ILs)‐based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission‐scanning electron microscopy (FE‐SEM), a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa (C. obtusa) by using ILs‐based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile‐water (25/75,V/V) as the mobile phase, and a flow rate of 0.7 mL·min?1. A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5–100.0 µg·mL?1. The recoveries ranged from 101.6% to 104.6% and the inter‐ and intra‐day relative standard deviations (RSD) were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants. |
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Keywords: | extraction ionic liquids monolithic myricetin quercetin |
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