Effect of Temperature on Self‐Interaction of Human‐Like Collagen |
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| Authors: | Daidi Fan Jianyu Xing Wenjiao Xue Chenhui Zhu Xiaoxuan Ma Rong Ma |
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| Institution: | 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, Shannxi 710069, China;2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, Shannxi 710069, China;3. School of Environmental Science and Engineering, Chang'an University, Xi'an, Shannxi 710054, China;4. Shaanxi Province Institute of Microbiology, Xi'an, Shannxi 710043, China |
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| Abstract: | Effects of temperature on self‐interaction of human‐like collagen (HLC) were investigated by hydrophobic interaction chromatography, calorimetric measurement, and sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) analysis. Results show that three types of interaction roles may exist between HLC molecules at 3–50°C, which were divided into three narrower temperature ranges. In temperature range from 3–22°C, hydrogen bonding plays a key role in the formation of a gelatinous aggregate. In the range of 22–38°C, hydrophobic bonds accompanied by hydrogen bonds are involved in the formation compact aggregates. When temperature is above 38°C the hydrophobic effect formed in the HLC monomer results in the loss of its ability to self‐interact. |
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| Keywords: | calorimetry human‐like collagen hydrophobic effect self‐interaction temperature range |
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