Live‐Cell Stimulated Raman Scattering Imaging of Alkyne‐Tagged Biomolecules |
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| Authors: | Senlian Hong Tao Chen Yuntao Zhu Ang Li Prof Yanyi Huang Prof Xing Chen |
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| Institution: | 1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Peking‐Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China);2. Biodynamic Optical Imaging Center (BIOPIC), College of Engineering, Peking University, Beijing 100871 (China) |
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| Abstract: | Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman‐silent region of a cell. Coupling this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label‐free microscopies. The bioorthogonal Raman imaging of various biomolecules tagged with an alkyne by a state‐of‐the‐art Raman imaging technique, stimulated Raman scattering (SRS) microscopy, is reported. This imaging method affords non‐invasiveness, high sensitivity, and molecular specificity and therefore should find broad applications in live‐cell imaging. |
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| Keywords: | biomolecules bioorthogonality Raman imaging stimulated Raman scattering |
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