|
|||||
|
|
Study of the effects of operational parameters on multiresidue pesticide analysis by LC-MS/MS |
|
| Authors: | Kmellár Béla Pareja Lucía Ferrer Carmen Fodor Péter Fernández-Alba Amadeo R |
| Affiliation: | a Pesticide Residue Research Group, EC Community Reference Laboratory for Pesicide Residues in Fruits and Vegetables Chemistry, University of Almeria, 04120, La Cañada de San Urbano, Almeria, Spain b Cornivus University of Budapest, Faculty of Food Science, Department of Applied Chemistry, 1118 Villany str. 29-43, Budapest, Hungary c Farmacognosia y Productos Naturales, Facultad de Quimica, General Flores 2124, Montevideo, Uruguay |
| Abstract: | In this paper, the influence of several operational parameters on a well established multiresidue LC-MS/MS method has been studied in relation to the analysis of 150 pesticides commonly present in vegetable samples. The operational parameters investigated are: (i) the influence of different modifiers (0.1% formic acid; 5 mM ammonium formiate; 5 mM ammonium acetate in aqueous phase) - both on the retention time and on the analytical response of the studied compounds; (ii) the effect of the analytical column's temperature on the retention time and on the analytical response of the pesticides investigated; (iii) the effects of co-elution in mixture containing 150 pesticides and, additionally, (iv) the carrying out of a study about the common transitions obtained by LC-MS/MS. Various common transitions were found among the 150 pesticides, but there were only two problematic cases, the pairs diuron-fluometuron and prometryn-terbutryn, which have common scanned transitions and have very close retention times. The use of ammonium salts as modifier instead of formic acid reports enhancement or suppression of the response depending on the pesticides. No great influence on the retention time or on the response of the pesticides and commodities studied was observed with relation to the column temperature. Two different columns: an HPLC (5 μm particle size) and an UHPLC analytical column (1.8 μm particle size) have been used. As was expected, shorter run times and lower peak width was achieved with the UHPLC column.In this paper, the effect of the compounds on each other in the MS analysis when the number of co-eluting compounds is quite high is also described. Mainly small suppression or enhancement co-elution effect was observed, but some particular pesticides presented high sensitivity (>±60% effect) when they elute together with others. This is an important factor and it has to be taken into account when performing multiresidue pesticide analysis. |
| Keywords: | LC-MS/MS Operational parameters Multiresidue methods Pesticides Food analysis |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |