| 气相色谱法测定胰酶中的丙酮溶剂残留量 |
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| 引用本文： | 林瑛,杨学灵,杨雪珠.气相色谱法测定胰酶中的丙酮溶剂残留量[J].广州化学,2013,38(2):34-36. |
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| 作者姓名： | 林瑛 杨学灵 杨雪珠 |
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| 作者单位： | 广州中科检测技术服务有限公司,广东广州 510650;中国科学院广州化学研究所分析测试中心,广东广州 510650 |
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| 摘 要： | 采用气相色谱测定了胰酶中残留的丙酮溶剂,并通过外标法定量分析其含量。气相色谱条件为:氢火焰离子化检测器(FID),DMF作为溶剂,DM-WAX色谱柱(30 m×0.53 mm×1μm),载气为氮气,进样口温度为200℃,检测器温度为300℃,柱温采取程序升温。气相色谱法检测丙酮的加标回收率为92.5%～98.0%,检出限(3S/D)为0.05μg/mL。
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| 关 键 词： | 气相色谱法 胰酶 丙酮 |
Determination of Acetone Residue in Pancreatin by Gas Chromatography |
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| LIN Ying
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YANG Xue-ling
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YANG Xue-zhu.Determination of Acetone Residue in Pancreatin by Gas Chromatography[J].Guangzhou Chemistry,2013,38(2):34-36. |
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| Authors: | LIN Ying YANG Xue-ling YANG Xue-zhu |
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| Institution: | (Guangzhou Zhongke Testing Services Co.,Ltd.,Guangzhou 510650,China;Analytical and Testing Center,Guangzhou Institute of Chemistry,Chinese Academy of Sciences,Guangzhou 510650,China) |
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| Abstract: | A method for the determination of solvent residue of acetone in pancreatin was established using gas chromatography. The solvent used in the determination was DMF, the column used was DB-WAX （30 mX0.53 mm×1utm）, the carrier gas used was nitrogen, the injector temperature was 200℃, the detector temperature was 300℃, and the column temperature was programmed in the determination process. Gas chromatography tested acetone and quantitatively analyzed by external standard method. Gas chromatography detection limit （3S/D） found for the determination of acetone was 0.05 ktg/mL and values of recovery found by standard addition method were in the range flom 92.5%~98.0%. |
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| Keywords: | gas chromatography pancreatin acetone |
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