| 人纤维蛋白溶酶原中色氨酸残基的化学修饰 |
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| 引用本文： | 赵陈光 邱爱东. 人纤维蛋白溶酶原中色氨酸残基的化学修饰[J]. 高等学校化学学报, 1997, 18(5): 744-747 |
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| 作者姓名： | 赵陈光 邱爱东 |
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| 作者单位： | 吉林大学分子生物学系!长春，130023(赵陈光，刘兰英)，卫生部长春生物制品研究所!长春，130062(邱爱东，刘淑英) |
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| 摘 要： | 以Ｎ－溴代琥珀酰亚胺为修饰剂，对人纤维蛋白溶酶原（ＨＰｇ）中色氨酸（Ｔｒｐ）残基的分布及其与酶活力的关系进行了研究，发现每个ＨＰｇ分子有１９个Ｔｒｐ残基，５个位于分子表面：有２个是快反应残基，其中１个是活性必需的氨基酸，酶被修饰后其荧光光谱及圆二色谱发生了变化。
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| 关 键 词： | 纤维蛋白溶酶原 色氨酸残基 化学修饰 HPg Trp |
Chemical Modification of Tryptophan Residues in Human Plasminogen |
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| ZHAO Chen-Guang, LIU Lan-Ying. Chemical Modification of Tryptophan Residues in Human Plasminogen[J]. Chemical Research In Chinese Universities, 1997, 18(5): 744-747 |
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| Authors: | ZHAO Chen-Guang LIU Lan-Ying |
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| Abstract: | Tryptophan(Trp) residues in human plasminogen(HPg) was modified by N-bro-mossuccinimide(NBS). The results indicate that there are 19 tryptophan residues in HPg.Five tryptophan residues are located on the surface of the enzymogen, two of them are fast reactive residues. Among all the Trp residues, one is essential for the activity of the enzyme and may be located at the substrate binding site of the enzyme. The spectra of circular dichroism(CD) of HPg are changed after treatment with NBS. |
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| Keywords: | Human plasminogen(HPg) Tryptophan(Trp) Chemical modification |
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