| 气相色谱-质谱联用测定紫花苜蓿中的硒蛋氨酸 |
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| 引用本文： | 陈国良,郑永杰,罗信义. 气相色谱-质谱联用测定紫花苜蓿中的硒蛋氨酸[J]. 广东微量元素科学, 2007, 14(5): 26-30 |
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| 作者姓名： | 陈国良 郑永杰 罗信义 |
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| 作者单位： | 齐齐哈尔大学,黑龙江,齐齐哈尔,161006;黑龙江省畜牧研究所,黑龙江,齐齐哈尔,161005 |
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| 基金项目： | 黑龙江省自然科学基金(No.B0314) |
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| 摘 要： | 建立了气相色谱质谱联用(GC—MS)测定紫花苜蓿中硒蛋氨酸含量的方法。将紫花苜蓿中提取的叶蛋白样品在6 mol/L HCl溶液中,超声处理20 min。离心分离后,以丁醇及三氟乙酸酐为衍生化试剂对硒蛋氨酸进行衍生化,采用选择离子监测模式对衍生物进行GC—MS测定。硒蛋氨酸的回收率为97.6%~105.2%,相对标准偏差为1.7%~3.6%,检出限为0.3 mg/L。该法样品前处理简单,测定快速,结果准确,灵敏度高,可用于植物样品中硒蛋氨酸含量的测定。
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| 关 键 词： | 气相色谱-质谱联用 硒蛋氨酸 紫花苜蓿 |
| 文章编号： | 1006-446X（2007）05-0026-05 |
| 收稿时间： | 2007-03-07 |
| 修稿时间： | 2007-03-07 |
Determination of Selenomethionine in Medicago sativa by Gas Chromatography- Mass Spectrometry |
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| CHEN Guoliang,ZHENG Yongjie,LUO Xinyi. Determination of Selenomethionine in Medicago sativa by Gas Chromatography- Mass Spectrometry[J]. Trace Elements Science, 2007, 14(5): 26-30 |
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| Authors: | CHEN Guoliang ZHENG Yongjie LUO Xinyi |
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| Affiliation: | 1. Qiqihar University, Qiqihar 161006, China; 2. Heilongjiang Animal Husbandry Institute, Qiqihar 161005, China |
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| Abstract: | A method has been developed for the determination of selenomethionine in Medicago sativa by gas chromatography-mass spectrometry(GC-MS).Leaf protein extracted from Medicago sativa was prepared with ultrasound in 6 mol/L HCl solution for 20 min.separated.The selenomethionine was derivatized with butanol and trifluoroacetic acid(TFA)after centrifuge.The derivatives were determined by GC/MS selectedion monitoring pattern.The recoveries of 97.6%~105.2% with relative standard deviations(RSDs) was of 1.7%~3.6% and the detection limit of selenomethionine for the method was 0.3 mg/L.The method was used for the determination of selenomethionine in plant samples has been proved to be rapid,high sensitivity,accurate and reliable. |
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| Keywords: | gas chromatography-mass spectrometry(GC-MS) selenomethionine Medicago sativa |
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