别嘌呤醇对黄嘌呤氧化酶的抑制作用研究

用聚苯胺黄嘌呤氧化酶电极（生物传感器）研究了别嘌呤醇对黄嘌呤对氧化酶的抑制作用，实验结果表明：别嘌呤醇对黄嘌呤氧化酶有明显的抑制作用，但这种抑制作用是可逆的，抑制剂在存在使固定酶的表观米氏常数增大但并没有影响催化反应的最大速率，所以别嘌呤是黄嘌呤氧化酶的一种可逆竞争抑制剂，抑制剂对固定酶的最适ｐＨ值没有影响，而对反应活化能略能影响。

Study on Inhibition of Xanthine Oxidase by Allopurinol

The investigation of inhibition of xanthine oxidase by allopurinol was carried out using a xanthine biosensor, which was obtained using the polyaniline film as a support material. The experimental results indicated that significant inhibition could be produced by allopurinol but this inhibition was reversible. The apprent Michaelis-Menten constant (K ) of the immobilized xanthine oxidase increased in the presence of allopurinol, but the maximum rate of the enzyme catalyzed reaction was not affected by allopurinol. This indicated that allopurinol is a reversible competitive inhibitor of xanthine oxidase. The optimum pH of the inunbilized enzyme was not thected, and the activation energy of the enzyme catalyzed reaction was slightly thected by allopurinol.