Lentivirus-mediated transgene delivery to the hippocampus reveals sub-field specific differences in expression |
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| Authors: | Lenneke WA van Hooijdonk Muhammad Ichwan Thomas F Dijkmans Theo G Schouten Marijke WA de Backer Roger AH Adan Fons J Verbeek Erno Vreugdenhil Carlos P Fitzsimons |
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| Affiliation: | (1) Medical Pharmacology Department, Leiden/Amsterdam Center for Drug Research, Leiden University Medical Center, Leiden University, The Netherlands;(2) Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, The Netherlands;(3) Leiden Institute of Advanced Computer Science, Section Imaging & BioInformatics, Leiden University, The Netherlands |
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| Abstract: | ![]()
Background In the adult hippocampus, the granule cell layer of the dentate gyrus is a heterogeneous structure formed by neurons of different ages, morphologies and electrophysiological properties. Retroviral vectors have been extensively used to transduce cells of the granule cell layer and study their inherent properties in an intact brain environment. In addition, lentivirus-based vectors have been used to deliver transgenes to replicative and non-replicative cells as well, such as post mitotic neurons of the CNS. However, only few studies have been dedicated to address the applicability of these widespread used vectors to hippocampal cells in vivo. Therefore, the aim of this study was to extensively characterize the cell types that are effectively transduced in vivo by VSVg-pseudotyped lentivirus-based vectors in the hippocampus dentate gyrus. |
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