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Gradient thin-layer chromatography of plant extracts
Authors:G. Matysik  E. Soczewiński
Affiliation:(1) Department of Inorganic and Analytical Chemistry, Medical Academy, Staszica 6, 20-81 Lublin, Poland
Abstract:
Summary A modified Niederwieser chamber for stepwise gradient elution, consisting of a PTFE capillary to store the series of eluents and a horizontal glass sandwich chamber with a glass distributor was used for the chromatographic separation of complex plant extracts (Seboren, Hemorigen and Pectosol) used in therapy. Densitograms demonstrate markedly improved separations of the extracts in comparison to isocratic elution.Produced by Polish Reagents, Melgiewska 18, 20-234 Lublin, Poland
Keywords:Thin-layer chromatography  Stepwise gradient elution  Chamber for gradient TLC  Densitometry of isocratic and gradient chromatograms
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