荧光碳点在脊椎类模式动物斑马鱼中的活体成像与毒理学研究

将荧光碳点引入透明斑马鱼胚胎中,研究了荧光碳点在斑马鱼中的活体成像和对斑马鱼的毒副作用。通过对水中添加荧光碳纳米颗粒,从形态上观察其对于斑马鱼个体发育的生物学效应,实时观察其在斑马鱼体内富集代谢的情况,为荧光碳点及其复合物在生物活体成像方面的研究应用奠定基础。结果表明,荧光碳点对斑马鱼生长发育无明显的毒副作用,不会导致胚胎发育异常(通过观察尾巴弯曲情况、色素深浅、血管发育速度、鱼鳔发育速度来确定对胚胎发育的影响),并且对斑马鱼无明显致死现象。荧光碳点进入斑马鱼体内的时间非常迅速,并且可以在48 h之内经代谢排出体外。

Flourescent Imaging and Toxicology Study of Carbon Dots in Transparent Zebrafishes

In this paper, we introduce the carbon dots (CDs) into transparent embryos of zebrafishes and study the fluorescent imaging and toxicology of CDs. With immerging oosperms and infants of zebrafishes into the aqueous solution of CDs for 1.5 h, the CDs go through the cell membrane and emerge inside of them. Then, from the morphological, we study the biological effects on the individual development and in vivo observe the supersession of zebrafishes. We demonstrate that CDs have no apparent toxicity on the growing development and do not induce the development abnormity of embryo. Furthermore, the CDs do not lead to the death of zebrafishes. We also find that CDs can enter into the zebrafisher rapidly and be expelled from the body within 48 h.