|
|||||
|
|
Kinetic properties of soluble and immobilizedCandida rugosa lipase |
|
| Authors: | Mar’ia D Virto ISabel Agud Sol Montero Alicia Blanco Rodolfo Solozabal Jos’e M Lascaray Mar’ia J Llama Juan L Serra L Carlos Landeta Mertxe De Renobales |
| Institution: | (1) Departamento de Bioqu’imica y Biolog’ia Molecular, Facultad de Farmacia, Uniuersidad del Pa’is Vasco/Euskal Herriko Unibertsitatea, Apartado 450, E-01080 Vitoria, Spain;(2) Departamento de Biotecnolog’ia, Inasmet, San Sebastian, Spain;(3) Departamento de Bioqu’imica y Biolog’ia Molecular, Facultad de Ciencias, Uniuersidad del Pa’is Vasco/Euskal Herriko Unibertsitatea, Bilbao, Spain;(4) Lascaray, S. A., Vitoria, Spain |
| Abstract: | Immobilized lipase (triacylglycerol ester hydrolase, EC 3.1.1.3) fromCandida rugosa has been immobilized on commercially available microporous polypropylene and used for the batch hydrolysis of different animal fats. The effect of the reaction products at concentrations similar to those obtained at 90% hydrolysis, both on soluble and immobilized lipase, was studied. Glycerol showed low inhibitory effect but oleic acid caused 50% inhibition. A mixture of free fatty acids present in the complete hydrolysis of beef tallow inhibited lipase activity more than 70%. The stability of the enzyme (both soluble and immobilized) was highest in the presence of 20% isooctane. The apparent Michaelis constant for each substrate for the soluble enzyme did not change on immobilization. |
| Keywords: | Lipase EC 3 1 1 3 immobilized enzyme animal fats hydrolysis organic solvents Candida rugosa kinetic parameters |
| 本文献已被 SpringerLink 等数据库收录！ | |