Sensitive label-free electrochemical immunoassay by electrocatalytic amplification |
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| Authors: | Chenrui Chen Dengyou Liu Zaisheng Wu Qimei Luo Guo-Li Shen Ru-Qin Yu |
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| Institution: | aState Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China;bScience College of Hunan Agricultural University, Changsha 410128, PR China |
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| Abstract: | A label-free electrochemical immunosensor for the detection of protein analytes without redox-active centers is for the first time developed based on the combination of gold nanoparticles and the mediated charge transport through the multilayer films that is related to an electrocatalytic process. Given the low detection limit, high sensitivity and selectivity, the success achieved here seems plausibly to serve as a significant step toward the development of versatile label-free immunoassay. |
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| Keywords: | Human immunoglobulin E (IgE) Methylene blue (MB) Gold nanoparticles (GNPs) |
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