甲磺酸培氟沙星荷移反应及荧光光谱性质研究

研究了甲磺酸培氟沙星与电子受体四氯对苯醌之间的荷移反应 ,实验表明 ,在甲醇体系中 ,甲磺酸培氟沙星与四氯对苯醌在光的作用下可形成稳定的荷移络合物 ,其荧光发射强度较甲磺酸培氟沙星显著增强 ,且最大激发波长和发射波长向长波方向分别位移 80和 6 0nm。在 0 0 5～ 10 μmol·L-1浓度范围内 ,甲磺酸培氟沙星荧光强度与浓度呈良好线性关系 ,其检出限为 0 0 0 4 μmol·L-1。该方法用于甲磺酸培氟沙星胶囊中甲磺酸培氟沙星含量的测定 ,结果与紫外 可见光度法一致。

Study on Fluorescence Characteristics of Pefloxacin Based on Charge Transfer Reaction

The charge transfer complex formed between pefloxacin mesylate (PM) as the donor and tetrachlorobenzoquinone (TCQ) as the acceptor has been studied by fluorimetry. Experiment shows that PM reacted with TCQ in methanol with light and the complex was formed. Its fluorimetric intensity was stronger than that of PM and the excitation and emission peaks of the complex were red shifted to 80 nm and 60 nm, respectively. A linear calibration graph was obtained over the PM concentration from 0.05 micromol x L(-1) to 10 micromol x L(-1) with the detection limit of 0.004 micromol x L(-1) of PM. The proposed method and UV-Vis photometric method were both applied to determining the pefloxacin in the same capsules with satisfactory results.