An amperometric urea biosensor based on covalent immobilization of urease on copolymer of glycidyl methacrylate and vinylferrocene |
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Authors: | Emre ?evik Mehmet ?enel Mustafa Fatih Abas?yan?k |
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Institution: | (1) Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul, 34500, Turkey;(2) Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul, 34500, Turkey;(3) Department of Chemistry, Fatih University, Buyukcekmece Kampusu, 34500, B.Cekmece, Istanbul, Turkey; |
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Abstract: | A unique urea biosensor construction based on the direct covalent attachment of urease onto a polymeric electron transfer
mediator, poly(glycidyl methacrylate-co-vinylferrocene)-coated electrode is described. Amperometric response was measured
as a function of urea concentration, at a fixed potential of +0.35 V vs. Ag/AgCl in phosphate-buffered saline (pH 7.0). Covalent
immobilization of the urease directly to the functionalized ferrocene copolymer surface produced biosensors with a short response
time (about 3 s) and provided low detection limits. The stability, reusability, pH, and temperature response of the biosensor,
besides its kinetic parameter, were also studied. |
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