A study for quality evaluation of Taxilli Herba from different hosts based on fingerprint-activity relationship modeling and multivariate statistical analysis |
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| Abstract: | In this study, a fingerprint-activity relationship modeling between chemical fingerprints and antirheumatic activity was established, and multivariate statistical analysis was used to evaluate the quality of Taxilli Herba (TH) from different hosts. Characteristic fingerprints of 20 batches of TH samples were generated by high-performance liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (HPLC-Triple TOF-MS/MS), and the similarity analysis was calculated based on thirteen common characteristic peaks by hierarchical clustering analysis (HCA). Subsequently, nine efficacy markers were discovered by combining fingerprints and antirheumatic activity through grey correlation analysis (GCA) and bivariate correlation analysis (BCA). Meanwhile, the content of 5 constituents in 9 markers was determined by high-performance liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (HPLC-QTRAP-MS/MS). The comprehensive quality of TH was assessed using multivariate statistical analysis, including principal components analysis (PCA) and technique for order preference by similarity to ideal solution (TOPSIS). The results showed that a high dose of TH extract could markedly ameliorate arthritis damage compared to other doses, with flavonoids playing an important role in the antirheumatic activity. The comprehensive quality of samples from Morus alba L. (SS) was superior to those from Liquidambar formosana Hance (FXS). The present study will demonstrate the markers associated with efficacy, and provide an applicable strategy for more comprehensive quality control and evaluation of TH. |
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| Keywords: | Taxilli Herba Hosts Fingerprint-activity relationship modeling Multivariate statistical analysis Quality evaluation AIA" },{" #name" :" keyword" ," $" :{" id" :" k0035" }," $$" :[{" #name" :" text" ," _" :" adjuvant-induced arthritis ANOVA" },{" #name" :" keyword" ," $" :{" id" :" k0045" }," $$" :[{" #name" :" text" ," _" :" one-way analysis of variance BCA" },{" #name" :" keyword" ," $" :{" id" :" k0055" }," $$" :[{" #name" :" text" ," _" :" bivariate correlation analysis BW" },{" #name" :" keyword" ," $" :{" id" :" k0065" }," $$" :[{" #name" :" text" ," _" :" body weight CMC-Na" },{" #name" :" keyword" ," $" :{" id" :" k0075" }," $$" :[{" #name" :" text" ," _" :" carboxymethyl cellulose sodium CUR" },{" #name" :" keyword" ," $" :{" id" :" k0085" }," $$" :[{" #name" :" text" ," _" :" curtain gas ELISA" },{" #name" :" keyword" ," $" :{" id" :" k0095" }," $$" :[{" #name" :" text" ," _" :" enzyme-linked immunosorbent assay ESI" },{" #name" :" keyword" ," $" :{" id" :" k0105" }," $$" :[{" #name" :" text" ," _" :" an electrospray ionization FCA" },{" #name" :" keyword" ," $" :{" id" :" k0115" }," $$" :[{" #name" :" text" ," _" :" freund’s complete adjuvant FHD" },{" #name" :" keyword" ," $" :{" id" :" k0125" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Liquidambar formosana" },{" #name" :" __text__" ," _" :" Hance high dose FLD" },{" #name" :" keyword" ," $" :{" id" :" k0135" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Liquidambar formosana" },{" #name" :" __text__" ," _" :" Hance low dose FMD" },{" #name" :" keyword" ," $" :{" id" :" k0145" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Liquidambar formosana" },{" #name" :" __text__" ," _" :" Hance middle dose FXS" },{" #name" :" keyword" ," $" :{" id" :" k0155" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Liquidambar formosana" },{" #name" :" __text__" ," _" :" Hance GCA" },{" #name" :" keyword" ," $" :{" id" :" k0165" }," $$" :[{" #name" :" text" ," _" :" grey correlation analysis GS1" },{" #name" :" keyword" ," $" :{" id" :" k0175" }," $$" :[{" #name" :" text" ," _" :" nebulization gas GS2" },{" #name" :" keyword" ," $" :{" id" :" k0185" }," $$" :[{" #name" :" text" ," _" :" auxiliary gas HCA" },{" #name" :" keyword" ," $" :{" id" :" k0195" }," $$" :[{" #name" :" text" ," _" :" hierarchical clustering analysis HPLC" },{" #name" :" keyword" ," $" :{" id" :" k0205" }," $$" :[{" #name" :" text" ," _" :" high performance liquid chromatography HPLC-QTRAP-MS/MS" },{" #name" :" keyword" ," $" :{" id" :" k0215" }," $$" :[{" #name" :" text" ," _" :" high-performance liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry HPLC-Triple TOF-MS/MS" },{" #name" :" keyword" ," $" :{" id" :" k0225" }," $$" :[{" #name" :" text" ," _" :" high-performance liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry IL-1β" },{" #name" :" keyword" ," $" :{" id" :" k0235" }," $$" :[{" #name" :" text" ," _" :" interleukin 1β IL-6" },{" #name" :" keyword" ," $" :{" id" :" k0245" }," $$" :[{" #name" :" text" ," _" :" interleukin 6 IL-10" },{" #name" :" keyword" ," $" :{" id" :" k0255" }," $$" :[{" #name" :" text" ," _" :" interleukin 10 IACUC" },{" #name" :" keyword" ," $" :{" id" :" k0265" }," $$" :[{" #name" :" text" ," _" :" Institutional Animal Care and Use Committee IS" },{" #name" :" keyword" ," $" :{" id" :" k0275" }," $$" :[{" #name" :" text" ," _" :" spray voltage LOD" },{" #name" :" keyword" ," $" :{" id" :" k0285" }," $$" :[{" #name" :" text" ," _" :" detection limit LOQ" },{" #name" :" keyword" ," $" :{" id" :" k0295" }," $$" :[{" #name" :" text" ," _" :" quantification limit PCA" },{" #name" :" keyword" ," $" :{" id" :" k0305" }," $$" :[{" #name" :" text" ," _" :" principal components analysis RA" },{" #name" :" keyword" ," $" :{" id" :" k0315" }," $$" :[{" #name" :" text" ," _" :" rheumatoid arthritis RSD" },{" #name" :" keyword" ," $" :{" id" :" k0325" }," $$" :[{" #name" :" text" ," _" :" relative standard deviation SHD" },{" #name" :" keyword" ," $" :{" id" :" k0335" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Morus alba" },{" #name" :" __text__" ," _" :" L. high dose SLD" },{" #name" :" keyword" ," $" :{" id" :" k0345" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Morus alba" },{" #name" :" __text__" ," _" :" L. low dose SMD" },{" #name" :" keyword" ," $" :{" id" :" k0355" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Morus alba" },{" #name" :" __text__" ," _" :" L. middle dose S/N" },{" #name" :" keyword" ," $" :{" id" :" k0365" }," $$" :[{" #name" :" text" ," _" :" signal-to-noise ratio SPF" },{" #name" :" keyword" ," $" :{" id" :" k0375" }," $$" :[{" #name" :" text" ," _" :" Specific Pathogen Free-grade SS" },{" #name" :" keyword" ," $" :{" id" :" k0385" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Morus alba" },{" #name" :" __text__" ," _" :" L. TCM" },{" #name" :" keyword" ," $" :{" id" :" k0395" }," $$" :[{" #name" :" text" ," _" :" traditional Chinese medicine TEM" },{" #name" :" keyword" ," $" :{" id" :" k0405" }," $$" :[{" #name" :" text" ," _" :" ion source temperature TH" },{" #name" :" keyword" ," $" :{" id" :" k0415" }," $$" :[{" #name" :" text" ," _" :" Taxilli Herba TGT" },{" #name" :" keyword" ," $" :{" id" :" k0425" }," $$" :[{" #name" :" text" ," _" :" tripterygium glycosides tablets TNF-ɑ" },{" #name" :" keyword" ," $" :{" id" :" k0435" }," $$" :[{" #name" :" text" ," _" :" tumor necrosis factor-alpha TOPSIS" },{" #name" :" keyword" ," $" :{" id" :" k0445" }," $$" :[{" #name" :" text" ," _" :" technique for order preference by similarity to ideal solution UV" },{" #name" :" keyword" ," $" :{" id" :" k0455" }," $$" :[{" #name" :" text" ," _" :" ultraviolet spectrophotometry |
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