Bioprospecting Dodonaea viscosa Jacq.; a traditional medicinal plant for antioxidant,cytotoxic, antidiabetic and antimicrobial potential |
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| Institution: | 1. Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan;2. Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, 44000 Islamabad, Pakistan;3. Department of Biology, Science Unit, Deanship of Educational Services, Qassim University, Buraidah 51452, Saudi Arabia;4. Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan;5. Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan;6. Department of Biochemistry, Science Unit, Deanship of Educational Services, Qassim University, Buraidah 51452, Saudi Arabia |
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| Abstract: | Purpose of studyDodonaea viscosa Jacq. is an ethnomedicinal plant that has been extensively used for the treatment of gout, rheumatism and pain. Current study was undertaken to mine its antioxidant, antimicrobial, cytotoxic and antidiabetic potential. Chromogenic assays were employed to establish plant’s multimode antioxidant profile whereas HPLC fingerprinting was performed to quantify polyphenols. Standard brine shrimp lethality, MTT and SRB assays proved its cytotoxicity potential.ResultsAmong all the extracts (flower, leaf, stem and root), maximum extract recovery (22% w/w), gallic acid equivalent total phenolic content (20.11 ± 0.11 ug GAE/mg DW), ascorbic acid equivalent total antioxidant capacity (22.5 ± 0.07 µg/mg DW) and total reducing power (31.1 ± 1.13 µg/mg DW) were recorded in the distilled water + acetone extract of leaf. The acetone extract of leaf showed maximum quercetin equivalent total flavonoid content (4.78 ± 0.13 µg/mg DW). HPLC-DAD analysis revealed significant amount of rutin, vanillic acid, coumaric acid, ferulic acid, gallic acid, syringic acid, cinnamic acid, gentisic acid, catechin, caffeic acid, apigenin and myricetin in the different plant parts. Maximum scavenging potential was exhibited by methanol + ethyl acetate stem extract (IC50 = 23.8 µg/ml). The highest antibacterial potential was found in flower (85.7%) and root (71.4%) extracts. The ethanol + ethyl acetate (1:1) leaf extract showed noteworthy toxicity against brine shrimps (LC50 = 95.46 µg/ml) while a notable antiproliferative activity against THP-1 (IC50 = 3.4 µg/ml) and Hep G2 (IC50 = 20 µg/ml) cell lines was shown by ethanol + ethyl acetate extracts (1:1) of stem and root, respectively. A moderate inhibition of α-amylase enzyme was observed in all parts of the plant.ConclusionThe results of the present study suggest D. viscosa as a potential source of antioxidant, anticancer and α-amylase inhibitory phytochemicals. |
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| Keywords: | Phytoconstituents Secondary metabolites Hep G2 hepatoma cell line THP-1 human leukemia cell line α-Amylase inhibition Antiproliferative activity DMSO"} {"#name":"keyword" "$":{"id":"k0045"} "$$":[{"#name":"text" "_":"Dimethyl sulfoxide TFC"} {"#name":"keyword" "$":{"id":"k0055"} "$$":[{"#name":"text" "_":"Total flavonoid contents TPC"} {"#name":"keyword" "$":{"id":"k0065"} "$$":[{"#name":"text" "_":"Total phenolic contents RSA: Radical scavenging activity DPPH"} {"#name":"keyword" "$":{"id":"k0075"} "$$":[{"#name":"text" "_":"2 2-diphenyl-1-picrylhydrazyl 50 % inhibitory concentration TAC"} {"#name":"keyword" "$":{"id":"k0095"} "$$":[{"#name":"text" "_":"Total antioxidant capacity TRP"} {"#name":"keyword" "$":{"id":"k0105"} "$$":[{"#name":"text" "_":"Total reducing power MIC"} {"#name":"keyword" "$":{"id":"k0115"} "$$":[{"#name":"text" "_":"Minimum inhibitory concentration Lethal concentration causing 50 % mortality ZOI"} {"#name":"keyword" "$":{"id":"k0135"} "$$":[{"#name":"text" "_":"Zone of inhibition |
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