| 多吡啶锌配合物键合与切割DNA研究 |
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| 引用本文： | 袁彩霞,魏毅斌,杨频. 多吡啶锌配合物键合与切割DNA研究[J]. 中国化学, 2006, 24(8): 1006-1012. DOI: 10.1002/cjoc.200690188 |
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| 作者姓名： | 袁彩霞 魏毅斌 杨频 |
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| 作者单位： | Institute of Molecular Science The Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry，Shanxi University，Taiyuan，Shanxi 030006，China.，Institute of Molecular Science，The Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry，Shanxi University，Taiyuan，Shanxi 030006，China.，Institute of Molecular Science，The Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry，Shanxi University，Taiyuan，Shanxi 030006，China. |
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| 基金项目： | Project supported by the National Natural Science Foundation of China(No.30470408)，Shanxi Provincial Natural Science Foundation. |
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| 摘 要： | The complex of Zn[(phen)(dione)Cl]ClO_4·H_2O(where phen is 1,10-phenanthroline and dione is 1,10-phenan-throline-5,6-dione)has been synthesized and characterized.The interaction of the complex with DNA was investi-gated using UV absorption,fluorescence spectroscopy and electrophoresis measurements.The results show that thecomplex mainly binds to the double helix of DNA with intercalation mode and the binding constant K is 2.4×10~4mol~(-1)·L.Moreover,the complex can efficiently cleave plasmid DNA at physiological pH and temperature.Thecleavage occurs via a hydrolysis mechanism,which is showed by adding radical scavengers,rigorously anaerobicexperiments,analysis for malondialdehyde-like products,and the hydrolysis experiment of BDNPP with a rate con-stant k_(obs)of 5.3×10~(-6)s~(-1).
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| 关 键 词： | zinc(Ⅱ)complex DNA binding hydrolytic cleavage |
| 收稿时间： | 2005-11-17 |
| 修稿时间： | 2005-11-172006-04-27 |
DNA‐Binding and Cleavage Studies of Zinc(II) Mixed‐polypyridyl Complex |
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| YUAN Cai-Xia, WEI Yi-Bin,YANG Pin. DNA‐Binding and Cleavage Studies of Zinc(II) Mixed‐polypyridyl Complex[J]. Chinese Journal of Chemistry, 2006, 24(8): 1006-1012. DOI: 10.1002/cjoc.200690188 |
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| Authors: | YUAN Cai-Xia WEI Yi-Bin YANG Pin |
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| Abstract: | The complex of Zn[(phen)(dione)Cl]ClO4·H2O (where phen is 1,10‐phenanthroline and dione is 1,10‐phenanthroline‐5,6‐dione) has been synthesized and characterized. The interaction of the complex with DNA was investigated using UV absorption, fluorescence spectroscopy and electrophoresis measurements. The results show that the complex mainly binds to the double helix of DNA with intercalation mode and the binding constant K is 2.4×104 mol?1·L. Moreover, the complex can efficiently cleave plasmid DNA at physiological pH and temperature. The cleavage occurs via a hydrolysis mechanism, which is showed by adding radical scavengers, rigorously anaerobic experiments, analysis for malondialdehyde‐like products, and the hydrolysis experiment of BDNPP with a rate constant kobs of 5.3×10?6 s?1. |
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| Keywords: | zinc(II) complex DNA binding hydrolytic cleavage |
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