Preparation of 125I-Lanreotide and its biodistribution in murine model of melanoma |
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| Authors: | U. Pandey A. Mukherjee G. Samuel S. Banerjee H. D. Sarma M. Venkatesh |
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| Affiliation: | (1) Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India;(2) Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India |
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| Abstract: | Lanreotide, a somatostatin analogue, was radioiodinated with 125I to explore the possibility of using 123I labeled lanreotide as a diagnostic radiopharmaceutical for tumors overexpressing somatostatin (SST) receptors. Radioiodination was carried out with 125I using chloramine T as the oxidant. The labeling yield was >90%. Characterization of 125I-Lanreotide was carried out by paper electrophoresis as well as HPLC. 125I-Lanreotide was purified by chromatography using a C18 Sep-Pak column. Radiochemical purity of the purified 125I-Lanreotide thus obtained was >99%. Significant tumor uptake of 125I-Lanreotide was observed in C57BL/6 mice bearing melanoma. |
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