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Covalent attachment of proteins to calcite surface for the single molecule experiments |
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| Authors: | Denisov N N Chtcheglova L A Sekatskii S K Dietler G |
| Affiliation: | aInstitute of Problem of Chemical Physics of the Russian Academy of Sciences, Semenov av. 1, 142432 Chernogolovka, Russia bMoscow Institute of Physics and Technology, Institutskii per. 9, 141700 Dolgoprudny, Moscow Region, Russia cLaboratoire de Physique de la Matière Vivante, IPMC, BSP, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland |
| Abstract: | Two protocols of covalent attachment of proteins onto the calcite surface, viz. one using the metallochelat and second using the aminohexil, are elaborated. Single molecule force spectroscopy method has been used to test their efficiency and practical applicability. Experiments were performed measuring the specific interaction force between bovine serum albumin (BSA) fixed onto the freshly cleaved calcite single crystal surface (procedure under the study here) and its polyclonal antibody (Ab-BSA) immobilized onto an AFM tip using standard and well studied procedure. We found the conditions, when up to 3–3.5% of tip-sample approaches lead to the formation of a single specific bond. |
| Keywords: | Calcite Single molecule Albumin Attachment AFM |
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