SINGLET OXYGEN FORMATION BY SENSITIZATION OF FUROCOUMARINS COMPLEXED WITH, OR BOUND COVALENTLY TO DNA |
| |
| Authors: | N J de Mol G M J Beijersbergen van Henegouwen B van Beele |
| |
| Institution: | Department of Pharmacochemistry, Subfaculty of Pharmacy, State University of Leiden, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands |
| |
| Abstract: | Abstract— The formation of singlet molecular oxygen (1O2) by sensitization of the furocoumarins 5-methoxypsoralen (5-MOP), 8-methoxypsoralen (8-MOP) and psoralen complexed with DNA was investigated. From the results it is concluded that 5-MOP complexed with native DNA is able to generate 1O2, even in a larger extent than 5-MOP free in solution. Also, with 8-MOP and especially with psoralen, 1O2 formation by the complexed compound could be observed. The 1O2 formation sensitized by covalently bound furocoumarin was demonstrated with psoralen as a model compound. 4',5'-Dihydropsoralen, a model compound for the UVA light absorbing 4',5'monoadducts of furocoumarins to DNA, is also able to generate 1O2. |
| |
| Keywords: | |
|
|
