Development and validation of an LC-MS/MS analysis for simultaneous determination of delphinidin-3-glucoside, cyanidin-3-glucoside and cyanidin-3-(6-malonylglucoside) in human plasma and urine after blood orange juice administration |
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| Authors: | Giordano Lucia Coletta Walter Rapisarda Paolo Donati Maria Benedetta Rotilio Domenico |
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| Institution: | Research Laboratories, John Paul II Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy. lucia.giordano@rm.unicatt.it |
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| Abstract: | Blood orange juice has a high content in anthocyanins, especially represented by delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G) and cyanidin-3-(6-malonylglucoside) (CMG). An LC-MS/MS method for the simultaneous determination of D3G and C3G in human plasma and urine was developed and validated. After sample preparation by SPE, chromatographic separation was performed with an RP-C(18) column, using a water/methanol linear gradient. The quantitation of target compounds was determined by multiple reaction monitoring (MRM) mode, using ESI. The method showed good selectivity, sensitivity (LOD = 0.05 and 0.10 ng/mL for C3G in plasma and urine, respectively; LOD = 0.10 ng/mL for D3G in plasma and urine), linearity (0.20-200 ng/mL; r >or= 0.998), intra- and interday precision and accuracy (
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| Keywords: | Blood orange juice Cyanidin‐3‐glucoside Cyanidin‐3‐(6‐malonylglucoside) Delphinidin‐3‐glucoside LC‐MS/MS |
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