Large injection volumes in capillary liquid chromatography: Study of the effect of focusing on chromatographic performance |
| |
Authors: | León-González M E Rosales-Conrado N Pérez-Arribas L V Polo-Díez L M |
| |
Institution: | Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avda. Complutense s/n, E-28040 Madrid, Spain. |
| |
Abstract: | This paper describes a multivariate approach to study the effect on chromatographic conditions and to optimize such conditions in capillary liquid chromatography when high injection volumes are required. Several separations have been evaluated by using isocratic and gradient solvent elution, as well as isocratic elution combined with temperature programming. In this study, easily ionisable organic compounds with low logP have been used as representative analytes. Injection volume and nature of the injection solution have been evaluated in order to increase the sensitivity (peak area) and column performance (N values). The equations obtained by multiple linear regressions and response surfaces allow achieving the optimum on-column focusing conditions for chlorophenoxy acids, carbamates and heterocyclic amines. |
| |
Keywords: | On-column peak focusing Large sample injection volumes Capillary liquid chromatography Chlorophenoxy acid herbicides Heterocyclic aromatic amines Carbamate insecticides |
本文献已被 ScienceDirect PubMed 等数据库收录! |